FIG. 1.

(A) Circular map of the Inc18-like vanA plasmid in the E. faecalis isolate from VRSA case 4. Genes are shown as arrows; certain partial or nonfunctional genes are shown as boxes. The putative conjugative transfer region is in gold, the vancomycin resistance genes are noted in light green, transposon genes are noted in dark blue, and the putative replication genes are in red. ORF, open reading frame. (B) Comparison of genetic organization of pWZ909 with that of Inc18 plasmids pRE25 and pIP501. The conjugative transfer region of pWZ909 is very similar to that of plasmids pRE25 from E. faecalis and pIP501 from Streptococcus agalactiae (24). The putative transfer region is shown in red with black outline, antibiotic resistance genes and replication regions are in blue, and transposon Tn1546 containing vancomycin genes is in red without outline.