FIG. 1.

Binding of 6W-RP-1 to vesicles composed of POPG and POPC (70:30 ratio). (A) Experimental binding kinetics recorded using 50 μM lipid and 0.5 μM peptide. The experimental data are the averages of five individual traces to improve the signal-to-noise ratio; the solid line is a single exponential fit to the data. (B) The apparent rate constant (k_app) was plotted against the lipid concentration to obtain k_on and k_off, as explained in the text. The error bars represent the standard deviations from five sets of experiments.