FIG. 2.

6W-RP-1 interaction with lipid vesicles as a function of the lysyl-DOPG content. The vesicle composition is based on a POPG:POPC ratio of 70:30, in which a fraction of the POPG was replaced by lysyl-DOPG. (A) The apparent rate constant, k_app, as a function of lysyl-PG content, measured at a lipid concentration of 100 μM. (B to D) The molecular rate constant for peptide binding, k_on (B), the molecular rate constant for 6W-RP-1 desorption, k_off (C), and the dissociation constant, K_D (D). The peptide concentration was 0.5 μM in all experiments. The error bars are the standard deviations for a minimum of two and usually three sets of experiments.