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Carboxyfluorescein leakage induced by 6W-RP-1 (5 μM) from vesicles (50 μM lipid) composed of POPG:POPC:lysyl-DOPG (70:30:0; dashed line), POPG:POPC:lysyl-DOPG (50:30:20; dotted line), POPG:POPC:lysyl-DOPG (40:30:30; dash-dotted line), and POPG:POPC:lysyl-DOPG (30:30:40; solid line). All curves are normalized to the maximum dye release, which was determined by adding Triton X-100 to the vesicles at a final concentration of 1% to dissolve the vesicles (1).
