TABLE 2.

Effects of environmental conditions and substrates on bactericidal activity of transferrins^a

Organism	Incubation conditions	Substrate (concn)	Cell viability (% of control)b
			rhLf	hTf
P. aeruginosa	Aerobic		18 ± 7	25 ± 6
	Anaerobic		95 ± 3	98 ± 2
	Anaerobic	KNO3(5 mM)	37 ± 9	52 ± 8
	Anaerobic	l-Arginine (40 mM)	92 ± 4	90 ± 2
L. lactis	Anaerobic		10 ± 3	96 ± 4c
	Anaerobic	Glucose (1%)	8 ± 4	93 ± 8c

^aP. aeruginosa or L. lactis cells were incubated (90 min) in 10 mM Tris-HCl (pH 7.4) with or without the indicated substrates under the specified environmental conditions.

^bValues are means ± SD for duplicates from at least three independent experiments. The IC₉₀s of lactoferrin (rhLf) and transferrin (hTf) were used.

^cAn hTf concentration of 25 μM was used.