Table 1.
Summary of NeuN+ area measurements indicating regional changes in hippocampal cell layers at different time points after ischemia.
| 10 days | 35 days | 90 days | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Area | Sham | 2VO | %Δ | Sham | 2VO | %Δ | Sham | 2VO | %Δ |
| DG Dorsal | 230,000 ± 19,920 | 214,100 ± 4,500 | −7% | 213,200 ± 30,410 | 255,600 ± 29,830 | ±20% | 230,800 ± 24,240 | 288,800* ± 50,390 | ±25% |
| DG Ventral | 193,100 ± 9,840 | 191,100 ± 27,360 | −1.4% | 178,800 ± 12,290 | 200,300 ± 11,870 | −12% | 200,800 ± 19,320 | 202,700 ± 7,410 | ±1% |
| CM Dorsal | 131,200 ± 20,160 | 8,200* ± 725 | −94% | 124,500 ± 9,410 | 1,500* ± 1,120 | −99% | 124,700 ± 7,240 | 8,900* ± 12,950 | −93% |
| CM Ventral | 56,900 ± 7,640 | 45,600* ± 13,790 | −20% | 83,700 ± 12,760 | 38,600* ± 5,340 | −54% | 68,900 ± 6,570 | 39,800* ± 26,900 | −42% |
Mean areas (μm2) and standard deviations are shown for each group. *indicates statistically significant difference between sham and 2VO groups. 2 way ANOVA with n = 3 in each group taking total time and treatment as variables. Area measurements (μm2) were done using Image J software as detailed in methods.