Fig. 1.
Chromatographic mobilities of hog barn dust (HBD) chloroform extract (HBDCHCl3) precipitates (HBDorg). A and B: 5 μl from 5 mg/ml solutions of HBDorg spotted on a thin-layer silica gel plate and developed for 45 min in solvents containing mixtures of CHCl3/MEOH with either triethylamine (A) or acetic acid (B). The plates were air-dried, visualized under 365-nm wavelength light, and photographed. Rf values were determined by measuring the distance the compound moved from the origin relative to the distance moved by the solvent. C: 10 μl from 5 mg/ml solution of HBDorg and 10 μl from 1 g/10 ml solution of HBD aqueous extract (HBDaq) spotted on a thin-layer silica gel plate alongside ryanodine (10 μl from 10 mg/ml).