Skip to main content
. Author manuscript; available in PMC: 2010 Sep 23.
Published in final edited form as: Mol Cell. 2000 Oct;6(4):861–871. doi: 10.1016/s1097-2765(05)00077-8

Figure 3. The Transmembrane Protein PfCRT Migrates as an Mr 45K Band and Localizes to the Digestive Vacuole.

Figure 3

(A) FLAG-specific M2 mAb recognizes epitope-tagged PfCRT as a Mr 45K protein in transformed 33-1/FLAG trophozoites. Rabbit IgG specific to the PfCRT-K peptide also recognize a protein of the same Mr in trophozoites from the 34-1/Erm line and in DV from the 34-1/Erm and 33-1/FLAG lines.

(B–D) Rabbit anti-PfCRT-K IgG specifically stain the DV in both 34-1/Erm (B and C) and 33-1/FLAG (D) parasites. Parasite location within the erythrocytes is evident in the differential interference contrast images (upper panels). Arrows indicate margins of the imaged intraerythrocytic parasites. Green fluorescence localizes PfCRT to the DV around the hemozoin pigment.