Fig. 5.

Ca²⁺ channel currents in isolated ganglion cells were reduced by L-803,087. A: examples of Ca²⁺ channel currents recorded from an isolated ganglion cell under voltage-clamp in the absence (left) and presence (right) of L-803,087 (10 nM). Voltage command protocol shown below. B: mean data show that administration of L-803,087 significantly reduced Ca²⁺ channel current at intermediate membrane potentials (n = 4; *P < 0.05, 2-way repeated-measures ANOVA vs. control). C: in the absence of L-803,087, administration of Ca²⁺ channel blockers, NIF (10 μM) and ω-conotoxin GVIA (CTX, 1 μM) reduced Ca²⁺ channel currents compared with control recordings (recorded at −10 mV, n = 3–4 cells/group). D: following administration of NIF, L-803,087 had no additional blocking effect on Ca²⁺ channel current, whereas following administration of CTX, L-803,087 further reduced Ca²⁺ channel current (−10 mV, n = 3–7 cells/group; **P < 0.05, ANOVA vs. L-803,087).