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. 2010 Sep 1;107(38):16432–16437. doi: 10.1073/pnas.1006044107

Fig. 2.

Fig. 2.

Attachment of the ATRP initiator (ABMP) to the C terminus of GFP. (A) SDS-PAGE analysis of cleavage of GFP from Mxe-ELP with a mixture of MESNA and ABMP. Lane 1: protein marker; Lane 2: GFP-Mxe-ELP fusion; Lane 3: GFP-Mxe-ELP fusion after cleavage; Lane 4: GFP-Br after purification by ITC. (B) Log10 intensity of fragment peptides after cleavage by Endo-C protease Blue column: GFP-SO3H; red column: GFP-Br. Note: Log10 = 5 is the approximate S/N limit, (C) Experimental isotope distribution of the C-terminal peptide K.LAAALGSGSGMRM-Br of GFP-Br, wherein M was oxidized.