Fig. 1.

Brain size analysis of Aspm mutant and human ASPM transgenic mice. (A and B) Brains from newborn (P0.5) and adult (9 wk) WT and Aspm^1-7-hom. Dashed lines delimit the rostrocaudal extent of the WT cerebral cortex (Cx). OB, olfactory bulb; Mb, midbrain; Cb, cerebellum. (Scale bar, 3 mm.) (C and D) Boxplot of whole body (C) and brain (D) weight of P0.5 WT and Aspm^1-25 (1-25) and Aspm^1-7 (1-7) heterozygous (het) and homozygous (hom) mice. Data from (left to right) 51, 34, 58, 18, and 22 mice. Mean weights compared with WT are indicated as percentages. (E) Double immunofluorescence for Brn1 (green) and FoxP2 (red) of vibratome sections (6-μm optical sections) of P0.5 neocortex. Cortical layers are indicated by II-VI. (Scale bar, 50 μm.) (F and G) Boxplot of whole-body (F) and brain (G) weight of adult (8–12 wk) WT, 1-25, and 1-7 het and hom, Aspm^1-7-hom with human ASPM BAC (1-7 hom +Hs), and WT mice with human ASPM BAC (+Hs). Data from (left to right) 65, 37, 27, 35, 59, 42, and 24 mice. Mean weights compared with WT are indicated as percentages. (H and I) Nissl staining of coronal 50-μm vibratome sections of adult brains from WT and 1-7 hom. Dashed lines delimit the lateral extent of the WT sections. Neocortex is highlighted in blue. Note the smaller size of the 1-7 hom brain. (Scale bar, 2 mm.) (J and K) Quantification of whole-brain (J) and neocortex (K) area across 15 sections along the rostrocaudal axis of control (WT and 1-7 het, n = 9, blue), 1-7 hom (n = 20, red), and 1-7 hom +Hs (n = 12, green). Sections in H and I are representative of sections 1 and 15 in the area quantifications, respectively. Data points indicate mean area (square millimeters); error bars indicate SEM. All data points of the 1-7 hom are significantly reduced (P < 0.001) compared with control. In boxplots (C, D, F, and G), the line within the box indicates the median value, the box spans the interquartile range, and whiskers extend to data extremes. *P < 0.05; **P < 0.01; ***P < 0.001.