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. 2010 Aug 31;107(38):16607–16612. doi: 10.1073/pnas.1004664107

Fig. 1.

Fig. 1.

H2-O−/− cells preferentially populate the GC. (A) Schematic representation of experimental setup. Mice were transferred with a 1:1 mixture of congenically marked B-8 and B1-8.H2-O−/− splenocytes and immunized with NP-CGG. Analysis was performed on days 0–21. (B) Contribution of transferred B1-8 and B1-8.H2-O−/− cells to donor-derived NP+ B-cell population over time, gated as shown in Fig. S1A. Numbers indicate percentage of each gated population. (C) Quantification of plots shown in B for multiple mice and experiments. (D) Ratio of B1-8.H2-O−/− to B1-8 B cells, normalized to ratio present in transferred mixture for data shown in B. (E) Contribution of transferred B1-8 and B1-8.H2-O−/− cells to donor-derived NP+ GC B-cell populations; gated as shown in Fig. S1A. (F) Quantification of plots shown in E for multiple mice and experiments. (G) Ratio of B1-8.H2-O−/− to B1-8. GC B cells, normalized to ratio in transferred mixture for data shown in E. Statistical significance was determined by two-tailed paired t test between B1-8 and B1-8.H2-O−/−.