Fig. 1.
Modeling of the chimerical DNA polymerase. The figure represents the structural model of a (HhH)2 domain (colored in cyan) joint through a linker peptide (in dark blue) to the C terminus of φ29 DNA polymerase (colored in gray). φ29 DNA polymerase TPR2 insertion and thumb subdomain are colored in pink and dark blue, respectively. The modeled primer, template, and displaced strands are colored in red, yellow, and green, respectively. The upstream tunnel that encircles the newly synthesized dsDNA, the downstream template tunnel, as well as the N and C termini of φ29 DNA polymerase are indicated. Crystallographic data are from Protein Data Bank ID codes 2PZX (φ29 DNA polymerase binary complex) (18) and 1BPX [(HhH)2 domain of DNA polymerase β (residues 91–148) (39)]. Figure was made by using the Swiss-PdbViewer software (www.expasy.org/spdbv).