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Chimerical DNA polymerases have an improved DNA binding. The 5′-labeled hybrid molecule 15mer/21mer (dsDNA) was incubated with φ29 DNA polymerase or with the indicated chimerical DNA polymerase, under the conditions described in Materials and Methods. After gel electrophoresis, the mobility of free dsDNA and the polymerase-DNA complex was detected by autoradiography. The experiment shown is representative of several experiments.
