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. 2010 Sep 7;107(38):16506–16511. doi: 10.1073/pnas.1011428107

Fig. 3.

Fig. 3.

Chimerical DNA polymerases show an enhanced RCR efficiency. (A) Strand displacement-coupled M13 DNA replication by φ29 DNA polymerase and chimerical DNA polymerases. Replication of 250 ng of singly primed M13 DNA was carried out as described in Materials and Methods using 60 nM of φ29 wild-type or chimerical DNA polymerases. The position of unit-length M13 DNA is shown at the right. (B) Multiply primed RCR of M13 DNA by φ29 DNA polymerase and chimerical DNA polymerases. The assay was performed as described in Materials and Methods, in the presence of 250 ng of multiply primed M13 DNA as input and 60 nM of either φ29 DNA polymerase or the indicated chimerical DNA polymerase. Data are represented as Mean ± SD. (C) Processive synthesis by φ29 DNA polymerase and chimerical DNA polymerases. The assay was performed as described in the text in the presence of 250 ng of singly primed M13 DNA and decreasing concentrations of the indicated DNA polymerase. After incubation at 30 °C for 20 min, samples were processed as described in A.