Fig. 5.

Multiply primed whole genome amplification of genomic DNA with φ29 DNA polymerase and chimerical DNA polymerases. The assay was performed as described in Materials and Methods, in the presence 1 ng of genomic DNA from B. subtilis and 50 nM of each polymerase. After the indicated reaction times, 1 μl of each sample was loaded in a Etd bromide-containing 0.7% agarose gel (A). At the left, linear DNA fragments obtained after digesting φ29 DNA with HindIII, used as DNA length markers. 1 μl of a 250-fold dilution of each sample was subjected to qPCR as described in Materials and Methods. The specific amplification factors are represented as Mean ± SD in B.