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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Microsc Microanal. 2010 Jun 22;16(4):478–487. doi: 10.1017/S1431927610000322

Figure 3.

Figure 3

Comparison of autofluorescence properties in living and fixed macrophage cells. A: Spectral signatures recovered from MCR analysis of hyperspectral imaging data. B: MCR extracted concentration map of macrophage autofluorescence in fixed cells. C: MCR extracted concentration map of macrophage autofluorescence in a separate set of living cells. D: MCR extracted concentration map of LysoTracker Red fluorescence from the same cells shown in C. The spectra in A were derived from images in B, C, and D. Scale bar = 5 μm. Intensity scales have been adjusted for display purposes. 61 × 84 mm (300 × 300 dpi).