Figure 6. In vitro cell proliferation assay validates CoPub Discovery's prediction.

The predicted influence of damnacanthal (red line and bullets) and dephostatin (blue line and triangles) on cell proliferation was tested in an in vitro cell assay. For both damnacanthal and dephostatin, the percentage of inhibition was measured and plotted against compound concentration. Both compounds were shown to inhibit cell proliferation in PBMCs when using concentrations in the 1 to 10 µM-range. The EC₅₀ was estimated at 1.96 µM for dephostatin and for damnacanthal at 2.67 µM.