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. 2010 Sep 23;6(9):e1001132. doi: 10.1371/journal.pgen.1001132

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Xu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Dense, cauliflower-like growth patterns of dcaf26^KO, cul4^KO, ddb1^KO, and dim-5 ^KO strains on plates with minimal media (30°C, 32h). (B) Growth rate of the wild-type, ku70^RIP, dcaf26^KO, cul4^KO, ddb1^KO, dim-5^KO, and dim-2^KO strains, measured at 25°C using the racetube assay in constant darkness. (C) Loss of histone H3K9 trimethylation at ku70^RIP, ζ-η, and ψ63 regions in the dcaf26^KO strain. Levels of H3K9 trimethylation at the indicated locations were determined by ChIP assay. Myc antibody was used as the negative control, and H3 antibody was used as the positive control and as the control for integrity of the nucleosome structure. (D) Western blot analysis of global H3 and H3K9 trimethylation in the wild-type strain and mutants.