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. 2010 Sep 23;6(9):e1001114. doi: 10.1371/journal.ppat.1001114

Figure 3. Identification of methylation-dependent Zta binding to selected viral promoter elements in vivo and in vitro.

Figure 3

Deep sequencing data obtained from in vitro immunoprecipitation experiments with GFP:BZLF1 as in Figure 1 are plotted on selected genes and their promoters as indicated. EBV DNA free of CpG-methylation (blue) and after full methylation by the methyltransferase M.SssI (red) were used as probes in the experiments. In vivo ChIP-seq data obtained after immunoprecipitation of chromatin of Raji cells (black) or B95.8 cells (green) with GFP:BZLF1 are plotted. Ten genes, their exon compositions and the location of selected ZREs are shown. (A) The two annotated genes BSLF2 and BMLF1 are shown, which result in the spliced BMLF2/BSLF1 transcript encoding the viral SM protein also called EB2. (B) The BFRF1 gene is shown. (C) The BALF2 gene is shown, which encodes the major DNA binding protein of EBV.