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. 2010 Sep 23;6(9):e1001118. doi: 10.1371/journal.ppat.1001118

Figure 3. Putative proline substrates of CyPA in NS5A domain II.

Figure 3

(A) Sequence of NS5A domain II. Each proline was changed to alanine in both WT and the DEYN background, using the J6-JFH genome. Mutant RNAs were electroporated into both Huh-7.5 sh-Luc and sh-A161 cell lines, and luciferase assays were performed at the indicated time points. The prolines were grouped into three categories according to phenotype. Representative replication kinetics of the mutants are shown in (B) for group I, (C) for group II and (D) for group III. The replication profiles of a GND replication defective mutant is shown in (E). (F) DEYN mutations fail to alleviate the lethal phenotype of double (P310AP315A) or quadruple prolines mutants. Error bars in (B), (C), (D), (E), and (F) represent standard derivations of two to three independent experiments.