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. 2010 Sep 23;6(9):e1001117. doi: 10.1371/journal.ppat.1001117

Figure 6. HBZ-specific CD8+ T cells are directly detectable ex vivo.

Figure 6

(A) CD4-depleted PBMC from ACs and HAM/TSP patients produce IFNγ in response to HBZ peptide. CD4-depleted PBMC were cultured in the presence of HBZ peptide. After 6hrs IFNγ producing cells were detected by ELISpot. The threshold for a positive response to peptide was defined as greater than the mean plus two standard deviations of the number of spots in the medium only control. (B) CD8+ T cells from ACs and HAM/TSP patients degranulate in response to HBZ. PBMC were cultured in the presence of anti-CD107a and monensin, in the presence of 2 µM HBZ peptide or its solvent, acetonitrile. After 5h, cells were harvested and stained. Dot plots shown are gated on the CD3+CD8+ live population.