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. 2010 Sep 23;6(9):e1001120. doi: 10.1371/journal.ppat.1001120

Figure 8. Inhibitory effects of the soluble TcpC TIR-domain on TLR signaling in murine macrophages.

Figure 8

(A) Wt BMDM were stimulated with Pam3Cys (1 µg/ml), poly(I:C) (2.5 µg/ml), ultrapure LPS from E. coli K12 (100 ng/ml), flagellin from S. typhimurium (1 µg/ml) and CpG 1826 (2 µM) in the presence of titrated amounts of the purified TIR domain of TcpC (TIR-TcpC) as indicated. TNF secretion was analyzed 3 hr after stimulation. (B) The experiment in (A) was repeated at the same time, side by side with Myd88−/− BMDM. Error bars represent SD from three individual cultures. The experiment was repeated once with similar results.