Figure 3. PP14·Fcγ1 pre-treated cells express the activation marker CD25.

PBMC from healthy donors were stimulated with MBP (20 µg/ml) in the presence or absence of PP14·Fcγ1 (50 µg/ml) or TGF-β (5 ng/ml) for two weeks. After two weeks the cells were restimulated with MBP for three days and then were collected and immunostained for the expression of CD25 on T cells (CD3⁺ cells).