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. 2010 Sep 23;6(9):e1001121. doi: 10.1371/journal.ppat.1001121

Figure 3. Internalized DiI-EBOV particles co-localize with the macropinosome marker sorting nexin (SNX) 5.

Figure 3

(A) Time-lapse analysis of the co-localization of DiI-labeled viral particles with eGFP-SNX5. DiI-EbolaΔVP30 virions (upper panels) or DiI-influenza virus (lower panels) were adsorbed to eGFP-SXN5-expressing Vero cells for 30 min on ice. The cells were then incubated at 37°C and time-lapse images were acquired at 20-second intervals over a period of 20 min by using confocal laser scanning microscope. Still frames at the indicated times (min) after the temperature shift to 37°C are shown. Virions co-localizing with SNX5 are indicated by arrows. Scale bars, 5 µm. (B) Co-localization efficiency of EBOV particles with SNX5. Shown are the co-localization efficiencies of DiI-EbolaΔVP30 (blue bars), DiI-Ebola VLPs (yellow bars), and DiI-influenza virus (red bars) with eGFP-SXN5 at the indicated time points after the temperature shift to 37°C. The number of DiI-labeled virions co-localized with eGFP-SNX5-positive vesicles was measured in 10 individual cells and the percentage of co-localization in the total DiI-virions is shown for each time point. Each experiment was performed in triplicate and the results are presented as the mean ± SD.