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. 2010 Sep 23;6(9):e1001121. doi: 10.1371/journal.ppat.1001121

Figure 4. Internalized DiI-labeled EBOV particles are transported to endosomes.

Figure 4

(A) Internalized DiI-labeled Ebola virions are transported to Rab7-positive vesicles. DiI-Ebola virions were adsorbed to eGFP-Rab7-expressing Vero cells for 30 min on ice. The cells were then incubated at 37°C and images were acquired at the indicated time points. Shown are representative images at 0 (left panel) and 120 min (right panel) after the temperature shift. DiI-labeled virions that co-localize with Rab7-positive vesicles are indicated by arrows. Insets show enlargements of the boxed areas. Scale bars, 10 µm. (B) Co-localization efficiency of EBOV particles with Rab7-positive vesicles. The co-localization efficiencies of DiI-EbolaΔVP30 virions (blue bars) and -Ebola VLPs (yellow bars) with Rab7-positive vesicles were analyzed at the indicated time points. The number of DiI-labeled virions co-localized with eGFP-Rab7-positive vesicles was measured in 10 individual cells and the percentage of co-localization in the total DiI-virions is shown for each time point. Each experiment was performed in triplicate and the results are presented as the mean ± SD.