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. 2010 Sep 23;5(9):e12908. doi: 10.1371/journal.pone.0012908

Figure 1. Inhibition of Tubulin deacetylases impairs axon elongation.

Figure 1

(A) 2 DIV hippocampal neurons cultured for 48 hours in the presence or absence of TSA (100 nM), and 4 DIV hippocampal neurons treated with TSA from 48 to 96 hours. Neurons were stained with antibodies against MAP2 and Tau-1 to define the somatodendritic and axonal compartments. Scale bar  = 50 µm. (B) Percentage of 2 DIV neurons that develop an axon, when treated with TSA (100 or 200 nM) from 5 hours to 48 hours. Data represent the mean ± SEM of 3 independent experiments (300 neurons/experimental condition and experiment). *p<0.05, **p<0.01, paired t-test. (C) Mean axon length of 4 DIV hippocampal neurons treated with TSA (100 or 200 nM) from 48 to 96 hours. Data represent the mean ± SEM of 3 independent experiments (100 neurons/experimental condition and experiment). *p<0.05, paired t-test.