Table 2. The bacteria strains and plasmids used in this study.

Strain or plasmid	Description	Reference or source
Strains
PPD130/91	Wild-type Edwardsiella tarda, Kms, Colr. Amps	Ling et al. (2000)
ΔevpC	PPD130/91, in-frame deletion of evpC	Srinivasa et al. (2004)
Plasmids
pSA10	pKK177-3 derivative, lacI, Ampr	S. Altuvia
pSAevpC	pSA10 with evpC fragment	This study
pSA6His-evpC	pSA10 with evpC fragment and 6His tag at N terminus	This study
pSAevpCD4A	pSA10 with evpC fragment, D4 residue is substituted with Ala	This study
pSAevpC E15A	pSA10 with evpC fragment, E15 residue is substituted with Ala	This study
pSAevpC E26A	pSA10 with evpC fragment, E26 residue is substituted with Ala	This study
pSAevpC D4AE15A	pSA10 with evpC fragment, D4 and E15 residues are substituted with Ala	This study
pSAevpC D4AE26A	pSA10 with evpC fragment, D4 and E26 residues are substituted with Ala	This study
pSAevpC E15AE26A	pSA10 with evpC fragment, E15 and E26 residues are substituted with Ala	This study
pSAevpC D4AE15AE26A	pSA10 with evpC fragment, substitute residues D4, E15 and E26 residues are substituted with Ala	This study
pSAevpC 1–160	pSA10 with evpC fragment, with K161K162K163 deletion	This study
pETM	pET32 derivative, Ampr	Novagen
pETMevpC	pETM with evpC fragment and 6His tag at C terminus,	This study
pETMevpC D4AE15A	pETM with evpC fragment and 6His tag at C terminus, D4 and E15 residues are substituted with Ala	This study
pETMevpC D4AE26A	pETM with evpC fragment and 6His tag at C terminus, D4 and E26 residues are substituted with Ala	This study
pETMevpC E15AE26A	pETM with evpC fragment and 6His tag at C terminus, E15 and E26 residues are substituted with Ala	This study