Figure 7. Rigidity-dependent changes in morphology and migration correlate with rigidity-dependent cell proliferation.

A.) Micrographs of A549, MDA-MB-231, PC-3, and mPanc96 cells that were plated on 150 or 4800 Pa gel substrates for 20 hours. B.) Areas of cells that were plated for 20 hours on 150 or 4800 Pa gel substrates. Results show mean fold increase over an unspread cell ± SEM of at least 20 cells counted for each condition. C.) A549, MDA-MB-231, PC-3, and mPanc96 cells were plated for 2 hours, then filmed for an additional 18 hours. Mean cell velocity ± SEM in µm/hr was determined by tracing and measuring the paths of 15 cells per rigidity per cell line. * p<0.05.