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. 2010 Sep 23;5(9):e12964. doi: 10.1371/journal.pone.0012964

Figure 1. GPR54 is coupled to ERK MAPK pathway in WT MEFs.

Figure 1

Representative autoradiographs (A and C) and densitometric analyses (B and D) showing the expression of total and activated ERK1/2 in GPR54 overexpressing WT1 (β-arrestin-1 KO parent) and WT2 (β-arrestin-2 and 1/2 KO parent) MEFs following 10-minute treatment with increasing concentrations of Kp-10 (0–1000 nM). Representative autoradiograph (E) and densitometric analysis (F) showing the expression of total and activated ERK1/2 in GPR54 overexpressing WT1 and WT2 parental MEF cell lines following 100 nM Kp-10 treatment (for the indicated time points: 0, 5, 10, 30 and 60 minutes). Western blot analyses were done using monoclonal anti-ERK1/2 and anti-phospho ERK1/2 antibodies. The data represent the mean ± S. E. of 4 independent experiments. *P<0.05; **P<0.01vs control (0 min.).