Skip to main content
. 2010 Sep 23;5(9):e12964. doi: 10.1371/journal.pone.0012964

Figure 3. GPR54 stimulation of ERK1/2 is enhanced in the absence of β-arrestin-1.

Figure 3

Representative autoradiograph (A) and densitometric analysis (B) showing the expression of total and activated ERK1/2 in GPR54 overexpressing β-Arr1 KO and corresponding wild type parental (WT1) MEFs following stimulation with 100 nM Kp-10 (for the indicated time points). (C) Representative western blot confirming absence and presence of expression of FLAG-GPR54 in non-electroporated (NT) and FLAG-GPR54 overexpressing β-Arr1 KO and WT1 MEFs, respectively. Representative autoradiograph (D) and densitometric analysis (E) showing the expression of total and activated ERK1/2 following stimulation with 100 nM Kp-10 (for indicated time points) of FLAG-GPR54 overexpressing β-Arr1 KO MEFs co-transfected with either GFP vector (grey bars) or β-Arr1-GFP (white bars). All western blot analyses were done using monoclonal anti-ERK1/2 and anti-phospho ERK1/2 antibodies. Monoclonal anti-β-Arr1 and anti-DDK (FLAG) antibodies were also used. The data represent the mean ± S. E. of 4 independent experiments (B) or the mean ± S. E. of 3 independent experiments (E). # P<0.05, ## P<0.01 vs 0 min. control (within the specific cell line). *P<0.05, **P<0.01 vs respective wild-type (or ‘add-back’) control at the indicated time point.