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. 2010 Sep 23;5(9):e12964. doi: 10.1371/journal.pone.0012964

Figure 6. β-arrestin-2 and Gq/11 regulate the GPR54-dependent ERK1/2 activation in a rapid and temporally overlapping manner.

Figure 6

Representative autoradiographs and densitometric analyses showing the expression of total and activated ERK1/2 in GPR54 overexpressing (A) β-Arr1 KO and WT1 MEFs; (B) β-Arr2 KO and WT2 MEFs; and (C) Gq/11 KO and WT MEFs following stimulation with 100 nM Kp-10 (for the indicated time points). All western blot analyses were done using monoclonal anti-ERK1/2 and anti-phospho ERK1/2 antibodies. The data represent the mean ± S. E. of 3 independent experiments. # P<0.05, ##P<0.01 vs 0 min. control (within the specific cell line). *P<0.05 vs respective wild-type control at the indicated time point.