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. 2010 Sep 23;5(9):e12964. doi: 10.1371/journal.pone.0012964

Figure 9. GPR54-mediated gene expression in the hypothalamic cell line, GT1-7 is altered following reduced β-arrestin-1 and 2 expression.

Figure 9

(A) GT1-7 cell lines stably expressing shRNAs against β-arrestin-1 (line 712) and β-arrestin-2 (line 153) were created and β-arrestin expression was determined by western blotting. This data revealed that lines 712 and 153 expressed about 50% less β-arrestin-1 and -2 than the WT parental line expressing scrambled shRNA sequences. (B) Unsupervised heirarchical clustering showing alterations of gene expression in the GT1-7 lines (712 and 153) relative to wild type. ANOVA was used to determine those probesets changing significantly (P<0.05) at least 1.5 fold in 712 and/or 153 versus WT. This list was then subjected to heirarchical clustering using the average linkage algorithm and displayed as a heat map (representative samples shown). Red: high expressor, blue: low expressor. Color scale: log(BASE 2) normalized intensity.