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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Am J Med Genet A. 2010 Mar;152A(3):770–776. doi: 10.1002/ajmg.a.33317

Figure 3.

Figure 3

Expression of Ank protein containing the craniometaphyseal dysplasia (CMD) mutation. A. Immunoblot analysis of COS-7 cells transfected with a mammalian expression vector encoding wild type murine Ank and green fluorescent protein (GFP) (WT Ank-GFP), or Ank containing the CMD mutation and GFP (CMD Ank-GFP). Untransfected cells and the cells transfected with a vector expressing GFP alone were used as controls. B&C. Immunofluorescent labeling of cells transfected with WT-Ank or CMD-Ank construct demonstrated that WT-Ank was localized in both plasma membrane (→) and cytoplasm (*) (B), while CMD-Ank was detected only in the cytoplasmic compartment as indicated with arrows (C).