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ε-NH3+ of Lys4 is protected from fast exchange with the solvent in trypsin bound MCoTI-I. {15N,1H}-HSQC spectra of free (a) and trypsin-bound MCoTI-I (b) were collected at RT with the 15N-carrier position at 82 ppm and 15N rf field strengths of 5.2 kHz for 90° and 180° pulses and 1.2 kHz for composite decoupling during acquisition.
