Table 1.
Bacterial strains and plasmids used in this study.
| Strains or plasmids | Characteristics | Reference |
|---|---|---|
| Bacteria | ||
| S. aureus RN4220 | A restriction minus derivative of S. aureus strain 8325-4 | (Kreiswirth et al., 1983) |
| S. aureus 8325-4 | A laboratory strain of S. aureus cured of all the prophages | (Novick, 1991) |
| S. aureus SH1000 | S. aureus strain 8325-4 with functional RsbU | (Horsburgh et al., 2002) |
| lyt− | An autolysis-deficient mutant strain of S. aureus which produces only a single band in autolytic-activity gels (ErmR) | (Mani et al., 1993) |
| VKS1005 | S. aureus strain SH1000 with mutation in the lytM gene (TetR) | This study |
| VKS1006 | S. aureus strain 8325-4 with mutation in the lytM gene (TetR) | This study |
| VKS1007 | S. aureus lyt− strain with mutation in lytM gene (ErmR, TetR) | This study |
| VKS1008 | VKS1006 complemented with 2.2 kb lytM gene under its promoter (KanR, CamR) | This study |
| SH1000lytP-lacZ | lytM promoter-lacZ reporter fused in the chromosome of S. aureus SH1000 (ErmR) | This study |
| SH1000agr:lytP-lacZ | lytM promoter-lacZ reporter fused in the chromosome of S. aureus agr mutant of strain SH1000 (ErmR, TetR) | This study |
| E. coli JM109 | recA1 supE44 endA1 hsdr17 gyra96 relA1I thi Δ(lac-proAB) F′ (traD36 proAB+ lacIqΔM15) | (Yanisch-Perron et al., 1985) |
| E. coli BL21(DE3)pLysS | F−, ompT, hsdSB (rB−,mB−), dcm, gal, λ(DE3), pLysS (Camr) | (Promega) |
| Plasmids | ||
| pGEMT | Cloning vector for E. coli (AmpR) | Promega |
| pTZ18R | Cloning vector for E. coli (AmpR) | (Mead et al., 1986) |
| pCU1 | Shuttle vector (AmpR in E. coli and CamR in S. aureus) | (Augustin et al., 1992) |
| pAZ106 | Plasmid with a promoterless lacZ | (Chan et al., 1998) |
| pRSETA | An E. coli overexpression plasmid | Invitrogen |
| pTZ18R-lytM | Plasmid pTZ18R containing left and right flanking regions of the S. aureus lytM gene. | This study |
| pTZ-lytM-tetM | A 2.2 kb tetracycline resistance gene was used to replace the BamHI fragment of construct pTZ18R-lytM. | This study |
| pCU1-lytM | Plasmid pCU1 containing 2.2 kb fragment representing S. aureus lytM promoter and coding region | This study |
| pRSETA-lytM | Plasmid pRSETA containing S. aureus lytM coding region | This study |
TetR - tetracyclin resistant, CamR - chloramphenicol resistant, ErmR - erythromycin resistant, AmpR - ampicillin resistant.