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Correlation between the insertion size and age at onset (n = 89). Representative PCR screening for the SCA31 insertion (a). Agarose gel electrophoresis of PCR products before and after HaeIII digestion is shown. M GeneRulerTM 1-kb Ladder (Fermentas Life Sciences, Burlington, Canada). The size of the insertion is inversely correlated with the age at onset (b). For 5 of 94 patients, age at onset could not be clearly defined by medical interview
