Table 1.
Overview of the transgenic lines ordered according to T-DNA copy number
| Expression of the reporter genes |
|||||||
|---|---|---|---|---|---|---|---|
| 6 months |
1·5 years |
4·5 years |
|||||
| Line | T-DNA copy number | NPT II | GFP | NPT II | GFP | NPT II | GFP |
| R1 | 1 | R | ++ | R | ++ | R | ++ |
| R10 | 1 | R | ++ | R | ++ | R | ++ |
| R24 | 1 | R | + | R | + | R | + |
| R25 | 1 | R | ++ | R | ++ | R | ++ |
| R26 | 1 | R | ++ | R | ++ | R | ↓+ |
| R28 | 1 | R | ++ | R | ↓− | S | − |
| R15 | 2 | R | ++ | R | ++ | R | ++ |
| R16 | 2 | R | + | R | + | R | + |
| R19 | 2 | R | ++ | R | ++ | R | ++ |
| R31 | 2 | R | ++ | R | ++ | R | ++ |
| R18 | 4 | R | + | R | + | R | + |
| R22 | 4 | R | ++ | R | ↓+ | R | + |
| R23 | 4 | R | + | R | + | R | + |
| R5 | 5 | R | + | R | + | R | + |
| R21 | 5 | R | + | R | ↓− | S | − |
| R17 | 6 | R | + | R | ↑+ + | S | ↓− |
| R30 | 6 | R | ↓− | S | − | S | − |
Expression of the reporter genes (NPTII and GFP) was determined 0·5, 1·5 and 4·5 years from the beginning of transformation. NPTII expression was evaluated as resistance (R) or sensitivity (S) to kanamycin. Levels of GFP (determined on two parallel immunostained Western blots) were sorted into three categories indicating high, low and no expression, as shown with ++ , + and −, respectively. Arrows (↑ ↓ ) indicate a decrease or increase in expression as compared with the previous determination.