Figure 1.

Activation of coagulation and pulmonary fibrin deposition in untreated pneumococcal pneumonia. Levels of (a, c) thrombin-antithrombin complexes (TATc) and (b, d) fibrin degradation products (FDP) in (a, b) lung and (c, d) plasma 24 and 48 hours after induction of pneumococcal pneumonia in wild-type mice (white, n = 8) and mice heterozygous (light grey white, n = 8) or homozygous (dark grey white, n = 8) for the factor V Leiden mutation. Representative slides of lung fibrin staining (brown) 48 hours after induction of pneumococcal pneumonia in (e) wild-type mice, (f) mice heterozygous and (g) mice homozygous for the factor V Leiden mutation (original magnification × 100). (h) Quantitation of pulmonary fibrin content 24 and 48 hours after induction of pneumococcal pneumonia in wild-type mice (white white, n = 8) and mice heterozygous (light grey white, n = 8) or homozygous (dark grey white, n = 8) for the factor V Leiden mutation. Data are expressed as box-and-whisker diagrams depicting the smallest observation, lower quartile, median, upper quartile and largest observation. ** indicates statistical significance as compared to wild-type mice (P < 0.01 Kruskal-Wallis test followed by Dunn's multiple comparison test).