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. 2010 Aug 23;14(4):R157. doi: 10.1186/cc9232

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Copyright ©2010 Cobelens et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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SAH was induced in male Wistar rats. Rats were treated with IFN-β for four consecutive days starting at two hours after the induction of SAH. Seven days post-SAH total lung homogenates were prepared and analyzed for inflammatory markers. (a) MPO activity corrected for the amount of protein. (b-d) Expression of endothelial activation markers E-selectin, ICAM-1, and VCAM-1. (e-g) Expression of chemokines MIP-1α, MIP-2, and CINC-1. (h) Expression of TNF-α. (b-h) Data are normalized for the expression of β-actin and GAPDH. Sham: N = 4; Sham/IFN-β: N = 4; SAH: N = 7; SAH/IFN-β: N = 6. *** P < 0.001, ** P < 0.01, * P < 0.05; SAH vs sham or SAH/IFN-β vs SAH. MPO, myeloperoxidase; MIP, macrophage-inflammatory protein; CINC, cytokine-induced neutrophil chemoattractant.