Figure 5.

Transient nature of mitogen-activated protein kinase and nuclear factor-κB activation in response to flagellin. The phosphorylation state of extracellular related kinase (ERK) and inhibitor of kappaB alpha (IκBα) was evaluated by immunoblotting in organs from mice under baseline conditions (Control C) and from mice challenged with 5 μg of flagellin for 30 minutes or 6 hours. The phosphorylation noted at 30 minutes did not persist after 6 hours. Blots are representative of three independent experiments.