Fig. 6. Identification of sequences interacting with Ter³⁴⁴³¹⁴ by 4C analysis.

A, schematic diagram showing the major steps of 4C analysis, the key enzymes/manipulation used at each step are indicated (see the text for further explanation); B, representative agarose gel of the inverse PCR products from a representative 4C analysis that used Ter³⁴⁴³¹⁴ and Ter^344314-M7 as baits, the asterisks show faint minor products that were found to be nonspecific PCR products, the lanes marked Reb1 (WT) and Reb1 (M7) refer to products from cells expressing WT Reb1 protein and which contained the Ter³⁴⁴³¹⁴ and Ter^344314-M7 bait sequences respectively; C, sequences of the bait and the prey sites; the two prey sequences captured by 4C namely Ter^4680236 and Ter^4257637 are both located in chromosome I, the third major band was self ligated bait sequence which, as expected, is missing from the ligase minus control lane in B (the primer sequences are shown in supplementary data).