Skip to main content
. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Mol Cancer Ther. 2010 Aug 24;9(9):2536–2544. doi: 10.1158/1535-7163.MCT-10-0332

Figure 3.

Figure 3

Substitution of Ad3 hexon onto Ad5 capsid results in improved biodistribution in mice. A, Modification of Ad5 hexon decreases liver transduction in mice. C57BL/6 mice were injected with Ad vectors and at 36 hours organs were harvested and luciferase transgene expression was assayed. * = p-value of < 0.05. B, Substitution of Ad3 hexon onto Ad5 capsid results in improved tumor to liver transgene ratio. Athymic nude mice with skov3.ip1 flank tumors underwent tail vein injections with Ad vectors. Luciferase transgene transcript copy numbers in liver and tumors were measured by quantitative PCR and normalized for genomic β–actin DNA. Furthermore, comparison was made between infectivity enhanced vectors with substitution of Ad3 fiber knob domain (right grouping) compared to vectors with Ad5 fiber knob domain (left grouping). * = p < 0.05 compared to liver control, ** = p < 0.05 compared to tumor control.