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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: DNA Repair (Amst). 2010 Jul 21;9(8):914–921. doi: 10.1016/j.dnarep.2010.06.005

Fig. 3. Yeast strains used to address the impact of DSBs on MMS-induced mutations.

Fig. 3

The strains used to assess MMS-induced LHM were described previously [8]. Presented is the portion of yeast chromosome V containing URA3, CAN1, and LYS2 ORFs is shown along with the telomere (triangle) and centromere (oval). A self-generating DSB cassette [24-26] containing a hygromycin resistance gene, the I-SceI endonuclease ORF under the control of the inducible GAL1-promoter and the I-SceI cut site was placed either in LYS2 (DSB-cen) or URA3 (DSB-tel). The “no-DSB” control cassette placed in LYS2 contained a noncleavable I-SceI half-site (not shown). The CAN1 mutation reporter is located within either 3.4 kb (DSB-cen and no DSB) or 4.4 kb (DSB-tel) from the I-SceI sites. URA3 and LYS2 were inserted 30 kb and 34 kb away from the telomere, respectively, in the DSB-tel and DSB-cen constructs.