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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: DNA Repair (Amst). 2010 Jul 21;9(8):914–921. doi: 10.1016/j.dnarep.2010.06.005

Fig. 5. Mutated bases of can1 alleles induced by MMS.

Fig. 5

Every can1 allele is given an arbitrary mutant number (as shown on the Y- axis). The mutated bases of can1 alleles are distinguished by colored symbols (green – thymine, blue – guanine, red – cytosine, and black – adenine) and by positions in the CAN1 ORF. The mutated bases are in the coding sequence of CAN1 and correspond to the template strand for repair of a DSB-tel and the complementary strand for repair of a DSB-cen. Open symbols represent single deletions and filled symbols represent single base substitutions. Multiple mutations appearing in the same mutant are connected by brown lines. To provide easy readability, grey lines separate groups of 5 mutants. Mutants were grouped by the mutated bases, and then mutations were ordered from lower to higher positions in CAN1 when possible. The number (n) of sequenced mutations is shown and the details are summarized in Table S8. (A, C) The DSB-cen and (B, D) DSB-tel strains were incubated in galactose to induce a DSB for 6 hours and then treated with 0.1% (v/v) MMS for 30 and 15 minutes, respectively. “No-DSB” strains were incubated in galactose for 6 hours and then half the cells were exposed to MMS (E) and half were not (F).