Fig. 1.

Jun a 1 wild-type and mutant ORFs in the TMV vector pJL36 [15]. The left and right T-DNA borders (LB and RB) allow vector insertion into the plant chromosome following agroinfection. The CaMV 35S plant promoter allows production of a viral mRNA, which then replicates extensively in the cytoplasm. A viral subgenomic promoter drives Jun a 1 expression. The four Jun a 1 mutant insertions are shown below, including wild-type rJun a 1, His²⁰³ to Ala mutant rJun a 1, 38 amino acid N-terminal truncated rJun a 1 and 38 amino acid N-terminal truncation with His²⁰³ to Ala mutant rJun a 1. Black arrow = CaMV 35S promoter; gray box = ribozyme; black box = 35S terminator. TMV ORFs: replicase, movement protein (MP), coat protein (CP).