Fig. 6.

Effects of SOD, L-NAME, epicatechin and FeTTPs on the ratio of p-Akt/total Akt in wild-type EPC. EPC cultures were exposed to nLDL (10 μg/ml) or oxLDL (10 μg/ml), or oxLDL (10 μg/ml) in the presence of SOD (100 U/ml), L-NAME (0.5 mM), epicatechin (100 μM) or FeTPPs (2.5 μM) in serum-free medium. A control well was treated with serum-free medium alone. Density of the Akt and p-Akt bands was first corrected with respect to β-actin (not shown) and then the ratio of p-Akt/total Akt expression calculated. The ratio in the control group was assigned a value of 1.0. Data are presented as mean ± SD, n = 3; ⁺ p < 0.05 oxLDL versus nLDL or control; * p < 0.05 versus oxLDL; ** p < 0.01 versus oxLDL.