Fig. 7.

Effects of SOD, L-NAME, epicatechin and FeTPPs on nitrotyrosine expression and the ratio of the PI3K p85 and p110 subunits in wild-type EPC. EPC cultures were exposed to nLDL (10 μg/ml) or oxLDL (10 μg/ml), or oxLDL (10 μg/ml) in the presence of SOD (100 U/ml), L-NAME (0.5 mM), epicatechin (100 μM) or FeTPPs (2.5 μM) in serum-free medium. A control well was treated with serum-free medium alone. a The p110 Akt subunit was isolated by immunoprecipitation; the expression of p110 and nitrotyrosine was determined. Note the essential absence of nitrotyrosine. This set of Western blots is representative of 3 separate experiments. b The p85 Akt subunit was isolated by immunoprecipitation; the expression of p110 and nitrotyrosine were determined. Density of the nitrotyrosine and PI3K p85 subunit bands were first corrected with respect to β-actin and then the ratio of nitrotyrosine/p85 expression calculated. The ratio of the control group was assigned a value of 1.0. Data are presented as mean ± SD, n = 3; ⁺ p < 0.05 oxLDL versus nLDL or control; * p < 0.05 versus oxLDL; ** p < 0.01 versus oxLDL. c Density of the PI3K p110 and PI3K p85 subunit bands were first corrected with respect to β-actin and then the ratio of p85/p110 expression calculated. The ratio of the control group was assigned a value of 1.0. Data are presented as mean ± SD, n = 3; ⁺ p < 0.05 oxLDL versus nLDL or control; * p < 0.05 versus oxLDL.