Fig. 7.

Determination of cytokines in BALF. a The cytokines in BALF were tested by a cytokine antibody array and analyzed by Multi Gauge version 3.0 for quantification. The BALFs were collected and pooled from mice in the same treatment groups, i.e. 6 animals in the control group, 7 animals in the WT group and 7 animals in the KO group. The levels of sTNFR-1 and IL-12p40 were lower in KO mice than in WT mice. b TGF-β1 concentration in BALF. No significant difference was found between KO and WT mice (NS), but both showed statistically significantly (p < 0.05) higher TGF-β1 concentrations than controls. Immunostaining was done twice and the determination was done in triplicate.