Fig. 8.

Histological (a) and immunochemical (b) staining of lung specimens of OVA-treated WT and KO mice and of saline-treated WT control mice. a Representative sections stained with HE, PAS, Giemsa and EVG. The patterns suggest airway inflammation, goblet cell hyperplasia and airway fibrosis in OVA-treated lungs. Magnification: ×20 for all photographs. b Representative staining for HA, CD44, SHAP-HA complex and sTNFR1. Strong HA staining may be observed in the lungs of OVA-treated mice compared with the lung of a saline-treated WT mouse. In the staining for CD44, the lungs of OVA-treated WT and KO mice show similar staining levels; the lung of a saline-treated WT control shows weaker staining. Obvious SHAP-HA complex staining may be observed around the airways of an OVA-treated WT mouse, but there is no significant staining around those of a saline-treated WT mouse. There is no staining in the KO mouse lung. In the immunostaining for sTNFR1, the specimens were observed using a fluorescence microscope. sTNFR1 appears to be upregulated in an OVA-treated mouse, and the stain is stronger in the specimen from a KO mouse than in that from a WT mouse. Magnification: ×20 for all photographs.